Characterization of live influenza vaccine donor strain derived from cold-adaptation of X-31 virus.
Identifieur interne : 001496 ( Main/Exploration ); précédent : 001495; suivant : 001497Characterization of live influenza vaccine donor strain derived from cold-adaptation of X-31 virus.
Auteurs : Kwang-Hee Lee [Corée du Sud] ; Sang-Uk Seo ; Jae-Min Song ; Chung-Min Lee ; Hyun-Ah Kim ; Baik L. SeongSource :
- Vaccine [ 0264-410X ] ; 2006.
Descripteurs français
- KwdFr :
- Adaptation physiologique, Administration par voie nasale, Animaux, Anticorps antiviraux (sang), Basse température, Chiens, Embryon de poulet, Femelle, Infections à Orthomyxoviridae (), Infections à Orthomyxoviridae (virologie), Lignée cellulaire, Méthode des plages virales, Phénotype, Poids du corps, Poumon (virologie), Souris, Souris de lignée BALB C, Sous-type H2N2 du virus de la grippe A (immunologie), Sous-type H3N2 du virus de la grippe A (immunologie), Test ELISA, Tests de neutralisation, Vaccins antigrippaux (administration et posologie), Vaccins antigrippaux (effets indésirables), Vaccins antigrippaux (génétique), Vaccins antigrippaux (immunologie), Vaccins atténués (administration et posologie), Vaccins atténués (effets indésirables), Vaccins atténués (génétique), Vaccins atténués (immunologie), Virus de la grippe A (croissance et développement), Virus de la grippe A (génétique), Virus de la grippe A (immunologie), Virus de la grippe A (pathogénicité).
- MESH :
- administration et posologie : Vaccins antigrippaux, Vaccins atténués.
- croissance et développement : Virus de la grippe A.
- effets indésirables : Vaccins antigrippaux, Vaccins atténués.
- génétique : Vaccins antigrippaux, Vaccins atténués, Virus de la grippe A.
- immunologie : Sous-type H2N2 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués, Virus de la grippe A.
- pathogénicité : Virus de la grippe A.
- sang : Anticorps antiviraux.
- virologie : Infections à Orthomyxoviridae, Poumon.
- Adaptation physiologique, Administration par voie nasale, Animaux, Basse température, Chiens, Embryon de poulet, Femelle, Infections à Orthomyxoviridae, Lignée cellulaire, Méthode des plages virales, Phénotype, Poids du corps, Souris, Souris de lignée BALB C, Test ELISA, Tests de neutralisation.
English descriptors
- KwdEn :
- Adaptation, Physiological, Administration, Intranasal, Animals, Antibodies, Viral (blood), Body Weight, Cell Line, Chick Embryo, Cold Temperature, Dogs, Enzyme-Linked Immunosorbent Assay, Female, Influenza A Virus, H2N2 Subtype (immunology), Influenza A Virus, H3N2 Subtype (immunology), Influenza A virus (genetics), Influenza A virus (growth & development), Influenza A virus (immunology), Influenza A virus (pathogenicity), Influenza Vaccines (administration & dosage), Influenza Vaccines (adverse effects), Influenza Vaccines (genetics), Influenza Vaccines (immunology), Lung (virology), Mice, Mice, Inbred BALB C, Neutralization Tests, Orthomyxoviridae Infections (prevention & control), Orthomyxoviridae Infections (virology), Phenotype, Vaccines, Attenuated (administration & dosage), Vaccines, Attenuated (adverse effects), Vaccines, Attenuated (genetics), Vaccines, Attenuated (immunology), Viral Plaque Assay.
- MESH :
- chemical , administration & dosage : Influenza Vaccines, Vaccines, Attenuated.
- chemical , adverse effects : Influenza Vaccines, Vaccines, Attenuated.
- chemical , blood : Antibodies, Viral.
- genetics : Influenza A virus, Influenza Vaccines, Vaccines, Attenuated.
- growth & development : Influenza A virus.
- immunology : Influenza A Virus, H2N2 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A virus, Influenza Vaccines, Vaccines, Attenuated.
- pathogenicity : Influenza A virus.
- prevention & control : Orthomyxoviridae Infections.
- virology : Lung, Orthomyxoviridae Infections.
- Adaptation, Physiological, Administration, Intranasal, Animals, Body Weight, Cell Line, Chick Embryo, Cold Temperature, Dogs, Enzyme-Linked Immunosorbent Assay, Female, Mice, Mice, Inbred BALB C, Neutralization Tests, Phenotype, Viral Plaque Assay.
Abstract
A human influenza A virus X-31 (high-yielding strain) was cold-adapted for possible future use as live attenuated vaccine. Mutant influenza viruses were selected during successive serial passage in embryonated hens' eggs at progressively lower sub-optimal temperature (30, 27 degrees C followed by 24 degrees C). The cold-passaged mutant exhibited both temperature-sensitivity (ts) and cold-adapted (ca) phenotypes. The pathogenicity and immunogenicity of X-31 ca virus were studied in mice following intranasal inoculation. The mice did not show clinical signs even at high titer infection. Immunization of mice with X-31 ca virus elicited high titers of neutralizing antibody and provided complete protection against homologous and heterologous virus challenges. To assess the genetic stability, the X-31 ca virus was passaged at 37 degrees C in MDCK cells or inoculated into mice. Revertant virus was not found in the lungs of any of the mice and the supernatants of the MDCK culture. We conclude that the X-31 ca candidate vaccine virus exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for live attenuated vaccine and merits further evaluation at clinical level.
DOI: 10.1016/j.vaccine.2005.10.051
PubMed: 16343703
Affiliations:
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effects)</term><term>Influenza Vaccines (genetics)</term><term>Influenza Vaccines (immunology)</term><term>Lung (virology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>Orthomyxoviridae Infections (prevention & control)</term><term>Orthomyxoviridae Infections (virology)</term><term>Phenotype</term><term>Vaccines, Attenuated (administration & dosage)</term><term>Vaccines, Attenuated (adverse effects)</term><term>Vaccines, Attenuated (genetics)</term><term>Vaccines, Attenuated (immunology)</term><term>Viral Plaque Assay</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adaptation physiologique</term><term>Administration par voie nasale</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Basse température</term><term>Chiens</term><term>Embryon de poulet</term><term>Femelle</term><term>Infections à Orthomyxoviridae ()</term><term>Infections à Orthomyxoviridae (virologie)</term><term>Lignée 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qualifier="pathogénicité" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Orthomyxoviridae</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Administration, Intranasal</term><term>Animals</term><term>Body Weight</term><term>Cell Line</term><term>Chick Embryo</term><term>Cold Temperature</term><term>Dogs</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>Phenotype</term><term>Viral Plaque Assay</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Administration par voie nasale</term><term>Animaux</term><term>Basse température</term><term>Chiens</term><term>Embryon de poulet</term><term>Femelle</term><term>Infections à Orthomyxoviridae</term><term>Lignée cellulaire</term><term>Méthode des plages virales</term><term>Phénotype</term><term>Poids du corps</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Test ELISA</term><term>Tests de neutralisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A human influenza A virus X-31 (high-yielding strain) was cold-adapted for possible future use as live attenuated vaccine. Mutant influenza viruses were selected during successive serial passage in embryonated hens' eggs at progressively lower sub-optimal temperature (30, 27 degrees C followed by 24 degrees C). The cold-passaged mutant exhibited both temperature-sensitivity (ts) and cold-adapted (ca) phenotypes. The pathogenicity and immunogenicity of X-31 ca virus were studied in mice following intranasal inoculation. The mice did not show clinical signs even at high titer infection. Immunization of mice with X-31 ca virus elicited high titers of neutralizing antibody and provided complete protection against homologous and heterologous virus challenges. To assess the genetic stability, the X-31 ca virus was passaged at 37 degrees C in MDCK cells or inoculated into mice. Revertant virus was not found in the lungs of any of the mice and the supernatants of the MDCK culture. We conclude that the X-31 ca candidate vaccine virus exhibits the desired level of attenuation, immunogenicity, and protective efficacy required for live attenuated vaccine and merits further evaluation at clinical level.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><noCountry><name sortKey="Kim, Hyun Ah" sort="Kim, Hyun Ah" uniqKey="Kim H" first="Hyun-Ah" last="Kim">Hyun-Ah Kim</name><name sortKey="Lee, Chung Min" sort="Lee, Chung Min" uniqKey="Lee C" first="Chung-Min" last="Lee">Chung-Min Lee</name><name sortKey="Seo, Sang Uk" sort="Seo, Sang Uk" uniqKey="Seo S" first="Sang-Uk" last="Seo">Sang-Uk Seo</name><name sortKey="Seong, Baik L" sort="Seong, Baik L" uniqKey="Seong B" first="Baik L" last="Seong">Baik L. Seong</name><name sortKey="Song, Jae Min" sort="Song, Jae Min" uniqKey="Song J" first="Jae-Min" last="Song">Jae-Min Song</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Lee, Kwang Hee" sort="Lee, Kwang Hee" uniqKey="Lee K" first="Kwang-Hee" last="Lee">Kwang-Hee Lee</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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